Equation 4

From Sednterp
Revision as of 20:31, 8 August 2011 by Bill Bashir (talk | contribs) (Created page with "The reduced apparent molecular weight, σ, is directly available from sedimentation equilibrium: Equating equation 1 and equation 2, one obtains the fundamental equation ...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

The reduced apparent molecular weight, σ, is directly available from sedimentation equilibrium: Equating equation 1 and equation 2, one obtains the fundamental equation describing sedimentation equilibrium of a single, thermodynamically ideal protein: equation 4.

Equation4-a.GIF

where is the reduced apparent molecular weight (Ref. 14 25), c(r) is the weight concentration at radial position r, and the other terms are as described above or in the table of symbols. While may be determined from the slope of a graph of ln c as a function of , sedimentation equilibrium data is analyzed best by nonlinear least squares procedures, which can accommodate more complicated cases involving association and nonideality (Ref. 2 10 14 26).