Parameter

Theory

Form

Sigma σ

Equations for Sigma

Main

RPM

Equations for Sigma

Main

Temperature

Equations for Sigma

Main

Mr. molecular weight (can be entered or computed)

Equations for Sigma

Main

s*. sedimentation coeff.

Equations for s

Main

mg/ml: sample concentration

Converting s to s20w

Main

Vbar: partial specific volume

Calculating Molecular Weight

The Sample Section

pH



Buffer Components Form

ρ Density of Buffer

Calculating Molecular Weight

Buffer Components Form

η Viscosity

Calculating Molecular Weight

Buffer Components Form

δ1 Hydration

Limitations on Estimation of Hydration and Asymmetry

Results

a/b asymmetry

Limitations on Estimation of Hydration and Asymmetry

Results
